Purification and assay of kinesin from sea urchin eggs and early embryos.
نویسندگان
چکیده
This paper describes the procedures used to purify the microtubule motor, kinesin, from mitotic cells, namely sea urchin eggs and cleavage stage embryos, and describes methods for assaying its motor activity.
منابع مشابه
Boursin, a sea urchin bimC kinesin protein, plays a role in anaphase and cytokinesis.
We have isolated and characterized Boursin, a kinesin-related protein of the bimC family, from Paracentrotus lividus sea urchin eggs. Boursin is expressed at high levels in eggs and embryos during early cleavage stages. Boursin was found to be associated with different parts of the mitotic spindle from early prophase to telophase. Expression of a form of the protein predicted to act as a domina...
متن کاملEmbryonic, larval and juvenile development of tropical sea urchin, Diadema setosum
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the In...
متن کاملEffects of mercury on embryonic development and larval growth of the sea urchin Echinometra mathaei from the Persian Gulf
This study investigated the effects of increasing mercury (Hg) concentration on early developmental stages of sea urchin, Echinomethra mathaei, as a bioindicator. The toxicity test was carried out after the gamete released induction and fertilization in six concentrations of mercury within the range of 4, 8, 16, 32, 64 and 128 µg/L. Embryos samples were incubated for 30 h in control and test so...
متن کاملEmbryonic, larval and juvenile development of tropical sea urchin, Diadema setosum
Diadema setosum (Leske, 1778), is one of the common echinoids widely distributed in the Indo-West Pacific Ocean, where it occurs from the Red Sea, Persian Gulf and the east coast of Africa to Japan, Australia and Malaysia. To investigate the developmental basis of morphological changes in embryos and larvae, we documented the ontogeny of D. setosum in a controlled laboratory condition at the In...
متن کاملDetermination of developmental stages of embryo in the Sea Urchin, Echinometra mathaei
Sea Urchin is one of the most useful tools in developmental biology studies because this organism has the simplest kind of developmental stages. We aimed to determine developmental stages and timetable of Echinometra mathaei embryo (the species of Persian Gulf). The spawning of E. mathaei was induced by 0.5M KCl injection (1ml) into the coelomic cavity. After fertilization, embryos were placed ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of cell science. Supplement
دوره 14 شماره
صفحات -
تاریخ انتشار 1991