Analysis of Polyethylene Glycol (PEG) and a Mono and Di-PEGylated Therapeutic Protein Using HPLC and Q-TOF Mass Spectrometry
نویسنده
چکیده
PEGylated peptides and proteins generate signifi cant challenges for the detailed structural characterization of biotherapeutics, mainly due to polyethylene glycol (PEG) heterogeneity. This application note describes a method for analyzing PEG and PEGylated protein using an Agilent HPLC coupled to an Agilent 6520 Accurate Mass Q-TOF LC/MS System. A charge stripping agent (triethylamine) was used as a post column addition to aid in obtaining a simpler mass spectrum and hence a better interpretation of the results. The results showed signifi cant improvement in the mass spectrum quality for PEG and the PEGylated protein. Authors Ravindra Gudihal and Suresh Babu CV Agilent Technologies India Pvt. Ltd. Bangalore, India Ning Tang Agilent Technologies, Inc. Santa Clara, CA, USA Sundaram Palaniswamy, Umamaheshwari S and Suneel Basingi GanGagen Biotechnologies Pvt. Ltd. Bangalore, India
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