f 3 - LipoproteinQuantitationin CordBloodSpottedon FilterPaper : A
نویسندگان
چکیده
We describe an electroimmunodiffusion technique for measuring /3-lipoprotein in cord blood spotted on filter paper. A series of cord-blood samples, taken from 916 consecutive live-birth infants, was spotted directly onto filter paper and assayed for /3-lipoprotein. Eleven had abovenormal /3-lipoprotein. Of these 11, seven were tested two to six months later, along with their parents, for total cholesterol and triglyceride concentrations. Five infants had increased cholesterol values, and four parents of these infantshad eitherincreasedcholesterol or triglyceride values. We also measured f3-lipoprotein concentrations in 63 paired samples of dried cord-blood and three-day postdelivery blood specimens, routinely collected for phenylketonuria screening. We saw a significant correlation between results for the specimens, but detected no cases of increased /3-lipoprotein. /3-Lipoprotein assay in dried specimens of cord blood is simple, inexpensive, and potentially is useful in mass screening of newborns for familial type II and combined hyperlipidemia.
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