Simultaneous Estimation of Cefixime Trihydrate and Erdosteine in Pharmaceutical Dosage Form by Using Reverse Phase - High Performance Liquid Chromatography

A simple, precise, accurate and sensitive Reverse phase high performance liquid chromatographic (RPHPLC) method for simultaneous estimation of Cefixime trihydrate and Erdosteine in combined capsule dosage form have been developed and validated. Drugs were resolved on a HiQ Sil C8 column (25cm X 4.6mm, 5μm), utilizing mobile phase of TetraButyl Ammonium Hydroxide (0.1 N aqueous) pH adjusted to 6.5 with Orthophosporic acid (10% aqueous) : ACN in a ratio of 2:1. Mobile phase was delivered at the flow rate of 1.0 ml/minute. Ultra violet Detection was carried out at 254nm. Separation was completed within 11 minutes. Calibration curves were linear with correlation coefficient 0.998 and 0.997 over a concentration range of 2-22 μg/ml for Cefixime trihydrate and 333 μg/ml for Erdosteine respectively. Recovery was between 99.92-101.12 percent and 100.01-100.34 percent for Cefixime trihydrate and Erdosteine respectively. Method was found to be reproducible with relative standard deviation (R.S.D) for intra and interday precision to be <1.5% over the said concentration range.