Simultaneous Determination of Glucose, Maltose

نویسندگان

  • C. Carola C. Rojas
  • Baboo Nair
  • Adelina Herbas
  • Björn Bergenståhl
چکیده

An enzymatic method has been developed to simultaneously determine glucose, maltose and sucrose in tubers The principle of the method is based on an enzymatic cleavage of these disaccharides and specific measurement the resulting glucose using an enzymatic procedure described by Holm (3). The study comprises two parts; the first one includes modifications of the pretreatment method to minimize errors due to ethanol used to precipitate polymeric material interfering with the activity of α-glucosidase and invertase. In the second part the usability of the method has been probed by employing it on six samples of cassava that come from Chapare-Bolivia.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

An automated, saccharogenic method for determining serum amylase activity.

An automated adaptation of the Somogyl saccharogenic determination of serum amylase is described in which conventional AutoAnalyzer modules are used. Adequate sensitivity with short incubation is achieved by incorporating glucose oxidase and catalase in the substrate to destroy serum glucose during incubation. Maltose and other dialyzable oligosaccharides are measured with the alkaline copper-n...

متن کامل

Separation Efficiency of Glucose and Maltose from Industrial Effluent by Granular Activated Carbon

This paper describes the performance of Granular Activated Carbon (GAC) to adsorb and separate glucose and maltose solutes from the industrial effluents by adsorption process. In this study, the capability of Granular Activated Carbon (GAC) to adsorb glucose and maltose were experimentally examined. Commercial GAC (mesh 12-20), supplied by Sigma Aldrich company in...

متن کامل

Detection and Quantitative Determination of Small Amounts of Glucose in Mixtures Containing Maltose

In the analysis of hydrolytic cleavage products of glycogen or starch, such as occur in tissue extracts and in diastatic reaction products in general, we were confronted with the task of differentiating between glucose and maltose, both qualitatively and quantitatively. Our studies called for a method that permits the detection and quantitative determination of slight amounts, often but fractio...

متن کامل

Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae

BACKGROUND In mixed sugar fermentations with recombinant Saccharomyces cerevisiae strains able to ferment D-xylose and L-arabinose the pentose sugars are normally only utilized after depletion of D-glucose. This has been attributed to competitive inhibition of pentose uptake by D-glucose as pentose sugars are taken up into yeast cells by individual members of the yeast hexose transporter family...

متن کامل

Tunable external cavity quantum cascade laser for the simultaneous determination of glucose and lactate in aqueous phase.

A room temperature operated pulsed external-cavity (EC) quantum cascade laser (QCL) was used for mid-infrared (mid-IR) transmission measurements of glucose and lactate in aqueous solution. The high spectral power density of the EC-QCL (ranging from 1-350 mW) over a wide tuning range (1030-1230 cm(-1)) allowed transmission measurements through optical paths of 130 μm and more. This is a signific...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007