Polytopic membrane protein folding at L 17 in the ribosome tunnel initiates cyclical
نویسنده
چکیده
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 195 No. 1 55–70 www.jcb.org/cgi/doi/10.1083/jcb.201103118 JCB 55 Correspondence to Arthur E. Johnson: [email protected] Abbreviations used in this paper: A, acceptor-containing sample; B, sample lacking acceptor and donor; D, donor-containing sample; DA, sample containing donor and acceptor; Ac-Lys, N-acetyl-lysine; ANB-Lys, N-(5-azido-2-nitrobenzoyl)lysine; BOF-Lys, N-BODIPY FL-lysine; BOP-Lys, N-BODIPY 576/589lysine; NBD-Lys, N-6(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl-lysine; pPL, preprolactin; PMP, polytopic membrane protein; PTC, peptidyltransferase center; RNC, ribosome-nascent chain complex; RTC, ribosome-translocon complex; SSMP, single-spanning membrane protein; TMS, transmembrane segment; VSVG, vesicular stomatitis virus protein G. Introduction
منابع مشابه
Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon
Multi-spanning membrane protein loops are directed alternately into the cytosol or ER lumen during cotranslational integration. Nascent chain exposure is switched after a newly synthesized transmembrane segment (TMS) enters the ribosomal tunnel. FRET measurements revealed that each TMS is initially extended, but folds into a compact conformation after moving 6-7 residues from the peptidyltransf...
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