Serological Relationships among Tombusviruses
نویسندگان
چکیده
Serological differentiation indices (SDIs) based on serum titrations in agar-gel double diffusion tests were determined for all ten definitive tombusviruses known at presen t, namely artichoke mottled crinkle, carnation Italian ringspot, cymbidium ringspot, eggplant mottled crinkle, Moroccan pepper, pelargonium leaf curl, petunia asteroid mosaic, the BS3 and type strains of tomato bushy stunt, and tombus Neckar viruses. More than 2000 titrations using 222 antisera from 36 rabbits were done. The SDIs ranged from 1 to >9, and all intermediate values were found. Simple trigonometry and computer methods were used to classify the results. There was no correlation between the serological relatedness of the particles of these viruses and the homology of their genome nucleic acids previously assessed by hybridization tests. In this respect they resemble the tymoviruses but not the tobamoviruses. The electrophoret~c migration of the particles of 12 tombusviruses was studied in 1 ~ agarose con:taining 20 mM-phosphate buffer pH 7.0. Some serologically indistinguishable tombusvirus strains migrated at different rates.
منابع مشابه
Serology of Bacterionema matruchotii.
Schmidt, Jean M. (State University of Iowa, Iowa City) and Robert L. Richardson. Serology of Bacterionema matruchotii. J. Bacteriol. 83:584-589. 1962.-Serological relationships of Bacterionema matruchotii were studied by means of the complement-fixation reaction. A common complement-fixing antigen was demonstrated in 37 strains of this organism. Serological relationships to species in the gener...
متن کاملSerological relationships among feline caliciviruses.
A total of 46 strains of feline calicivirus isolates from the United Kingdom, United States, Australia, and New Zealand were used in an investigation of their serological relationships based on the serum neutralization test. Although demonstrable antigenic variation exists between these isolates, it is shown that significant in vitro cross-activity exists between all these isolates to greater o...
متن کاملCytoplasmic antigen relationships among the Actinomycetales.
Kwapinski, J. B. (The University of New England, Armidale, N.S.W., Australia). Cytoplasmic antigen relationships among the Actinomycetales. J. Bacteriol. 87:1234-1237. 1964.-Cytoplasm obtained from 44 strains of the Actinomycetales was tested against the homologous and heterologous antisera in a diffusion precipitation test. A pattern of serological relationships among the cytoplasmic fractions...
متن کاملSerological studies on Leuconostoc mesenteroides.
Following the recognition of four primary colonial types of Leuconostoc mewnteroies by McCleskey et al. (1947), Leiva-Quiros and McCleskey (1947) studied the serological relationships among strains of the four types. They employed glucose-grown cultures for the production of antibodies so the cell antigens could be studied without the complication introduced by the dextran hapten. Several worke...
متن کامل