Oxidative Metabolism of Phenylacetic Acid by Penicillium chrysogenum Q-176. Part 6. The Production of Homogentisic Acid by a Mutant Strain Induced from Pen. chrysogenum
نویسنده
چکیده
Penicillium chrysogenurn Q-176に よPhenylacetic Acidの 酸化的代謝 に關す る研 究(第6報) Pen. chyysognum Q-176 の1変 異 種 に よ るHomogentisic Acidの 生成 に つ い て (そ の2)Phenylacetic Acid代 謝 異 常 菌 株 の菌 学 的性 質 磯 野 正 雄(武田 薬品工業株式会社大阪工場) 昭 和28年10月24日 受 理 Oxidative Metabolism of Phenylacetic Acid by Penicillium chrysogenum Q-176. Part 6. The Production of Homogentisic Acid by a Mutant Strain Induced from Pen. chrysogenum Q-176. (2) The Microbioiogical Natures of the Mutant Strains. By Masao ISONO (Osaka Factory, Takeda Pharmaceutical Industries Ltd.)
منابع مشابه
Penicillium chrysogenum Takes up the Penicillin G Precursor Phenylacetic Acid by Passive Diffusion.
Penicillium chrysogenum utilizes phenylacetic acid as a side chain precursor in penicillin G biosynthesis. During industrial production of penicillin G, phenylacetic acid is fed in small amounts to the medium to avoid toxic side effects. Phenylacetic acid is taken up from the medium and intracellularly coupled to 6-aminopenicillanic acid. To enter the fungal cell, phenylacetic acid has to pass ...
متن کاملReduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains.
The single-copy pahA gene from Penicillium chrysogenum encodes a phenylacetate 2-hydroxylase that catalyzes the first step of phenylacetate catabolism, an oxidative route that decreases the precursor availability for penicillin G biosynthesis. PahA protein is homologous to cytochrome P450 monooxygenases involved in the detoxification of xenobiotic compounds, with 84% identity to the Aspergillus...
متن کاملIncreased penicillin production in Penicillium chrysogenum production strains via balanced overexpression of isopenicillin N acyltransferase.
Intense classical strain improvement has yielded industrial Penicillium chrysogenum strains that produce high titers of penicillin. These strains contain multiple copies of the penicillin biosynthesis cluster encoding the three key enzymes: δ-(l-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), isopenicillin N synthase (IPNS), and isopenicillin N acyltransferase (IAT). The phenylacetic aci...
متن کاملA study of lipides of Penicillium chrysogenum.
Relatively few studies have been made on the lipide metabolism of Penicillium chrysogenum, although large quantities of these compounds are used as antifoam agents in the production of penicillin. Goldschmidt and Koffler (1) demonstrated that unsaturated long chain fatty acids present in the antifoam were responsible for stimulation of penicillin yields, since many physiologically inert compoun...
متن کاملBiological breakdown of benzylpenicillin by preformed mats of penicillin-producing organisms.
1. Penicillium chrysogenum and Aspergillus flavus degraded benzylpenicillin in the same way. 2. Degradation of the antibiotic was initiated by the cleavage of phenylacetic acid from 6-aminopenicillanic acid. 3. Phenylacetic acid was left unchanged whereas 6-aminopenicillanic acid was degraded into cysteine and valine. This reaction is probably complex. 4. Cysteine was not utilized but valine wa...
متن کامل