Micellar hybrid nanoparticles for simultaneous magnetofluorescent imaging and drug delivery.

Experimental Section For the micellar hybrid nanoparticle (MHN) synthesis, 100 μL (MHN1), 300 μL (MHN3), or 500 μL (MHN5) of TOP-coated CdSe/ZnS or CdSexTe1-x/ZnS quantum dots (QD, Invitrogen, CA, USA) in chloroform (2 mg/mL), and 100 μL of oleic acid-coated magnetic iron oxide nanoparticles (MN, prepared using a previously reported method [1]) in chloroform (2 mg/mL), were mixed with 200 uL of 1,2-dipalmitoyl-sn-glycero-3phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG-PE or PEGphospholipids, Avanti Polar Lipids, Inc., AL, USA) in chloroform (10 mg/mL). For the drug-incorporated MHN, 100 uL of doxorubicin (DOX, Sigma-Aldrich Chemicals, MO, USA) in chloroform containing triethylamine (TEA) (molar ratio 1:1=TEA:DOX, 1 mg/mL) was also added. After complete evaporation of the chloroform, the dried film was hydrated by adding 1 mL water at 75 C and the synthesis vessel was placed in an ultrasonic bath for 5 min to obtain an optically clear suspension. The suspension was first filtered through a 0.1 μm membrane.