Studies on Succinic Dehydrogenase
نویسنده
چکیده
About 2 years ago the author reported that the activity of purified preparations of succinic dehydrogenase is enhanced by phosphate buffer (2). This enhancement was dependent upon the phosphate concentration and appeared to be relatively specific for phosphate, since other buffers, such as Tris,l imidazole, histidine, glycylglycine, and arsenate did not induce it (2, 3). Arsenate, in fact, interfered with the activation by phosphate in a competitive manner. Subsequent studies on the mechanism of the phosphate activation revealed that the activation was elicited by compounds capable of forming a complex with the active center of the enzyme and that it is characterized by a high energy of activation together with changes in the absorption spectrum and Michaelis constants of the enzyme suggestive of a configurational change in the protein (1). The present paper is a detailed account of these findings.
منابع مشابه
Studies on succinic dehydrogenase activity of gastric mucosa in mice.
Succinic dehydrogenase is considered to be one of the most important enzymes that demonstrate the functional state of the parietal cell of gastric mucosa. Succinic dehydrogenase of the gastric mucosa was first done histochemically by Padykula with the parietal cell in rat. Thereafter many authors have described on the study of succinic dehydrogenase of the gastric mucosa and the relationship be...
متن کاملEFFECT OF ACTIVE MOLLUSCICIDAL COMPONENETS OF ABRUS PRECATORIUS, ARGEMONE MEXICANA AND NERIUM INDICUM ON CERTAIN ENZYMES IN THE NERVOUS TISSUE OF LYMNAEA ACUMINATA
In vivo and in vitro sublethal exposure of abrin, glycyrrhizin (active component of Abrus precatorius seed and root), sanguinarine, protopine (Argemone mexicana seed) and oleandrin (Nerium indicum leaf) inhibited the acetylcholinesterase, lactic dehydrogenase, acid and alkaline phosphatase activity in the nervous tissue of Lymnaea acuminata. The succinic dehydrogenase activity in the nervous ti...
متن کاملStudies on the Terminal Electron Transport System
The present communication is devoted to the description of the isolation, properties, and composition of a particulate unit which we shall refer to as the succinic dehydrogenase complex (SDC). The working hypothesis underlying this and subsequent communications in this series is that SDC is a unit of enzymatic action in the same sense as any well defined soluble enzyme. Since the discovery of s...
متن کاملEvidence of redox imbalance in a patient with succinic semialdehyde dehydrogenase deficiency
The pathophysiology of succinic semialdehyde dehydrogenase (SSADH) deficiency is not completely understood. Oxidative stress, mitochondrial pathology, and low reduced glutathione levels have been demonstrated in mice, but no studies have been reported in humans. We report on a patient with SSADH deficiency in whom we found low levels of blood reduced glutathione (GSH), and elevations of dicarbo...
متن کاملSuccinic Acid Monoethyl Ester and Metformin Regulates Carbohydrate Metabolic Enzymes and Improves Glycemic Control in Streptozotocin-Nicotinamide Induced Type2 Diabetic Rats
Objective. Succnic acid mono ethyl ester (EMS) was recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. In the present study the effect of EMS and Metformin on the activities of carbohydrate metabolic enzymes in streptozotocinnicotinamide induced type 2 diabeteic model was investigated. Methods. EMS were injected intraperitonially at doses 2...
متن کاملGenome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production.
Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, Mannheimia succiniciproducens, gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strai...
متن کامل