Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition
نویسندگان
چکیده
It has been hypothesized that the relatively low concentration of sulfur amino acids in legume seeds might be an ecological adaptation to nutrient poor, marginal soils. SARC1 and SMARC1N-PN1 are genetically related lines of common bean (dry bean, Phaseolus vulgaris) differing in seed storage protein composition. In SMARC1N-PN1, the lack of phaseolin and major lectins is compensated by increased levels of sulfur-rich proteins, resulting in an enhanced concentration of cysteine and methionine, mostly at the expense of the abundant non-protein amino acid, S-methylcysteine. To identify potential effects associated with an increased concentration of sulfur amino acids in the protein pool, the response of the two genotypes to low and high sulfur nutrition was evaluated under controlled conditions. Seed yield was increased by the high sulfate treatment in SMARC1N-PN1. The seed concentrations of sulfur, sulfate, and S-methylcysteine were altered by the sulfur treatment in both genotypes. The concentration of total cysteine and extractible globulins was increased specifically in SMARC1N-PN1. Proteomic analysis identified arcelin-like protein 4, lipoxygenase-3, albumin-2, and alpha amylase inhibitor beta chain as having increased levels under high sulfur conditions. Lipoxygenase-3 accumulation was sensitive to sulfur nutrition only in SMARC1N-PN1. Under field conditions, both SARC1 and SMARC1N-PN1 exhibited a slight increase in yield in response to sulfur treatment, typical for common bean.
منابع مشابه
Assessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]
Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti-nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS-PAGE and scanning densitometry. The results showed that seed storage protein ba...
متن کاملMethionine-containing proteins in two Phaseolus vulgaris cultivars with different methionine bioavailabilities.
The full utilization of legumes as human food is limited by a deficiency of sulfur amino acids, low protein digestibility, low methionine bioavailability and the presence of anti-nutritional factors. A new cultivar of Phaseolus vulgaris (Carioca 80) has 56.8% available methionine, compared with 29.3% availability in the parent cultivar Carioca. The total methionine content, denaturing gel elect...
متن کاملTranscriptomic analysis reveals differential gene expression in response to aluminium in 1 common bean ( Phaseolus vulgaris L . ) genotypes differing in aluminium resistance 2
متن کامل
The effect of Thiobacillus and Sulfur application on yield and some of physiological traits in common bean (Phaseolus vulgaris) cultivars under water deficit
A research has been conducted in research greenhouse of Islamic Azad University, Tabriz Branch in spring 2016 to investigate the effect of Sulfur and Thiobacillus on yield and some of physiological traits in common bean cultivars in experimental water deficit stress conditions as a factorial experiment in a completely randomized design with three replications. In this study investigated three v...
متن کاملUse of an in vitro Rumen Gas Production Technique to Evaluate the Nutritive Value of Five Forage to Concentrate Ratios
This study presents the chemical composition and in vitro fermentation of five diets with different forage (alfalfa) to concentrate (faba bean) (F:C) ratios, F0:C100, F25:C75, F50:C50, F75:C25, F100:C0 on a dry matter (DM) basis. Results indicate that the chemical composition in term of organic matter (OM), ether extract (EE), crude protein (CP), acid detergent fiber (ADF), neutral detergent fi...
متن کامل