A PCR ELISA Method For The Detection Of Yersinia Enterocolitica
نویسندگان
چکیده
and Implications The ELISA assay is a more rapid and sensitive method for PCR product detection than conventional gel electrophoresis. The PCR/ELISA allows for the processing of numerous samples, is relatively inexpensive and eliminates the need for electrophoretic and photographic equipment as well as the use of potential carcinogens such as ethidium bromide which is utilized in the gel electrophoretic detection of PCR products. The PCR ELISA is suitable for the rapid processing of samples required for field surveys of hogs and pork products.
منابع مشابه
Detection of Yersinia enterocolitica in Pigs and Pork Products
Introduction Yersinia enterocolitica is a zoonotic organism affecting both humans and livestock. In humans, Y. enterocolitica infection can result in severe abdominal pain, which mimics appendicitis. Swine have been implicated as the principal animal reservoir for human pathogenic Y. enterocolitica. Infections in pigs may localize in the tonsils (3). Several outbreaks of yersiniosis have involv...
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