Fellowships Grants and Awards
نویسندگان
چکیده
ette-Guérin (Mycobacterium bovis BCG) in protecting humans against tuberculosis has prompted a search for the mechanisms through which BCG induces chemokines. In this study, our experiments were designed to determine the role of the transcription factor nuclear factor-kB (NF-kB) and intracellular calcium in the production of interleukin (IL)-8, a main chemotactic factor, by human-derived monocytic cell line U937 and by a human epithelial HEp-2 cell line infected with M. bovis BCG. Methods : The concentrations of IL-8 in culture supernatants of U937 cells or HEp-2 cells infected with M. bovis BCG were determined by enzyme-linked immunosorbent assay. We used sulfasalazine and curcumin, which are well-described inhibitors of NF-kB activity, and we used ethylenediamine tetraacetic acid to deplete extracellular Ca or used the cell-permeable agent 1,2-bis (2-aminophenoxy) ethane-N ,N ,N ?,N ?-tetraacetic acid tetra (acetoxymethyl) ester to chelate releasable intracellular stores of Ca in order to investigate the mechanisms through which M. bovis BCG induces IL-8 secretion in our system. Results : The enzyme-linked immunosorbent assay showed that IL-8 protein secretion was elevated in M. bovis -infected cell lines. This effect was statistically significant (pB/ 0.01). When calcium influx was suppressed in M. bovis -infected cell lines, IL-8 secretion was inhibited. Notably, specific inhibitors of NFkB (sulfasalazine and curcumin) inhibited M. bovis induced IL-8 secretion from U937 cells or HEp-2 cells. Conclusions : Collectively, these results indicate that activation of NF-kB is an important signal transduction pathway in M. bovis -induced IL-8 secretion in monocytic or epithelial cells. Furthermore, the results showed that calcium influx had a direct effect on IL-8 secretion in U937 cells or HEp-2 cells infected with M. bovis .
منابع مشابه
Productivity outcomes for recent grants and fellowships awarded by the American Osteopathic Association Bureau of Research.
The objective of the present study was to evaluate productivity outcome measures for recent research grants and fellowships awarded through the American Osteopathic Association (AOA) Bureau of Research. Recipients of grants and fellowships that were awarded between 1995 and 2001 were contacted by mail, e-mail, or telephone and asked to provide information about publications, resulting grant awa...
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ورودعنوان ژورنال:
- Environmental Health Perspectives
دوره 110 شماره
صفحات -
تاریخ انتشار 2002