A prestalk-cell-specific acid phosphatase in Dictyostelium discoideum.

نویسنده

  • A A Oohata
چکیده

A simple and reliable method of separating and quantifying acid phosphatase activities in D. discoideum is described, and one of the electrophoretically distinguishable forms of the enzyme is shown to be specific for prestalk cells. Accumulation of this product commences some 2-3 h later in development than the prespore specific enzyme UDP-galactose polysaccharide transferase. This finding is discussed in terms of recent observation on the control of prestalk cell differentiation in this organism.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Use of a monoclonal antibody recognizing a cell surface determinant to distinguish prestalk and prespore cells of Dictyostelium discoideum slugs.

Double labelling experiments on Dictyostelium discoideum cells at different developmental stages were carried out using monoclonal antibodies MUD1 (prespore specific), MUD9 (strong label on prestalk and anterior-like cells) and a fluorescence-activated cell sorter. The monoclonal antibody MUD9, which recognizes the surface of prestalk and anterior-like cells strongly and prespore cells weakly, ...

متن کامل

Control of cell type proportions by a secreted factor in Dictyostelium discoideum.

It has been shown that, in Dictyostelium discoideum, conversion of prestalk cells to prespore cells in suspension cultures is inhibited by coexisting prespore cells. To examine whether the inhibition of conversion requires direct cell contact or is mediated by substances secreted by the cells, prestalk cells and prespore cells were incubated in shaken suspension, separated from each other by a ...

متن کامل

Separation and biochemical characterization of the two cell types present in the pseudoplasmodium of Dictyostelium mucoroides.

The pseudoplasmodium (slug) of the cellular slime mould, Dictyostelium mucoroides consists of prestalk and prespore cells. These 2 differentiated types of cells were separated by modification of the previous methods using density-gradient centrifugation. Major improvements made in the present study were the use of a density column of different specific gravities and the use of a discontinuous g...

متن کامل

Modelling cell movement, cell differentiation, cell sorting and proportion regulation in Dictyostelium discoideum aggregations.

Understanding the mechanisms that control tissue morphogenesis and homeostasis is a central goal not only in developmental biology but also has great relevance for our understanding of various diseases, including cancer. A model organism that is widely used to study the control of tissue morphogenesis and proportioning is the Dictyostelium discoideum. While there are mathematical models describ...

متن کامل

Chemotactic cell sorting in Dictyostelium discoideum.

We made mixed mounds of vitally stained prestalk cells and unstained prespore cells, from Dictyostelium discoideum (Dd), and exposed these to external 3',5'-cyclic-AMP (c-AMP) sources. We show that prestalk cells will sort out by moving directionally toward c-AMP sources held at less than 10(-5) M c-AMP. This is evidence for cell sorting via chemotaxis. We present arguments that the natural cel...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of embryology and experimental morphology

دوره 74  شماره 

صفحات  -

تاریخ انتشار 1983