Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation.

نویسندگان

  • Yanping Xu
  • Bo Zhang
  • Mark Messerli
  • Gerhard Randers-Pehrson
  • Tom K Hei
  • David J Brenner
چکیده

A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.

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عنوان ژورنال:
  • Radiation and environmental biophysics

دوره 54 1  شماره 

صفحات  -

تاریخ انتشار 2015