Quantifying the syncytialisation of a human placental trophoblast cell line grown in vitro
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چکیده
We have generated ‘green’ and ‘red’ BeWo cell lines: the green one constitutively expresses the green fluorescent protein (GFP) attached to histone H2B (Kimura & Cook, 2001), and the red one the red fluorescent protein tagged to the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mit-DsRed; Bevis & Glick, 2002). Our quantitative assay for cell fusion (syncytialisation) utilizes the fluorescence-activated cell sorter (FACS) to detect ‘cells’ that are both green and red.
منابع مشابه
Quantifying the syncytialisation of human placental trophoblast BeWo cells grown in vitro.
We have generated lines of BeWo cells that constitutively and stably express either histone H2B tagged with the green fluorescent protein (GFP), or the mitochondrial targeting sequence of subunit VIII of cytochrome c oxidase fused with a red fluorescent protein; one line has nuclei that fluoresce green, the other mitochondria that fluoresce red. Expression of these tagged proteins has no effect...
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Female and male reproductive tracts are of interest sites to study of immune system because they encounter specific infections such as those are sexually transmitted. Furthermore, female reproductive tract is in close contact with allogenic sperms and transmitted microorganisms during intercourse and semi allogenic fetus during pregnancy. In mammals, there are two types of immune responses, the...
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تاریخ انتشار 2002