Validated Spectrophotometric Methods for the Determination of Cefuroxime Axetil in Bulk Drug and Tablets

Two simple spectrophotometric methods have been developed for the determination of Cefuroxime Axetil in bulk drug and its tablet formulation. Method I is a simple UV spectrophotometric method. In this method the simple UV spectrum of Cefuroxime Axetil in 0.1 N HCl was obtained which exhibits absorption maxima (λ max) at 281 nm. Calibration curve was prepared by plotting the absorbance vs. concentration. The quantitative determination of the drug was carried out at 281 nm and the linearity range was found to be 2 to 30 μg/ml. Method II is the 1 derivative spectrophotometric method. In this method the simple UV spectrum of Cefuroxime Axetil in 0.1 N NaOH was obtained and derivatised to 1 order. Maxima occur at 266 nm and minima at 300 nm. A calibration curve was prepared by plotting the absorbance difference between maxima and minima vs. concentration. The linearity range was found to be 4 – 30 μg/ml. All the proposed methods were extensively validated. The proposed methods were successfully applied to the assay of Cefuroxime Axetil in pure and tablet dosage form. No interference was found from tablet excipients at the selected wavelengths and assay conditions. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations.