Amylase production by thermomonospora curvata.
نویسندگان
چکیده
Thermomonospora curvata produces an extracellular inducible amylase which does not accumulate products repressive to cellulase production during growth on starch-cellulose ratios similar to those of compost.
منابع مشابه
Production, purification, and characterization of alpha-amylase from Thermomonospora curvata.
Thermomonospora curvata produces an extracellular alpha-amylase. Maximal amylase production by cultures in a starch-mineral salts medium occurred at pH 7.5 and 53 degrees C. The crude enzyme was unstable to heating (65 degrees C) at pH 4 to 6, and was activated when heated at pH 8. The enzyme was purified 66-fold with a 9% yield and appeared homogeneous on discontinuous gel electrophoresis. The...
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A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced ...
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A catabolite repression-resistant mutant of the thermophilic actinomycete Thermomonospora curvata was obtained by treatment with ethyl methanesulfonate and UV light. Cellulase biosynthesis was undiminished by glucose, 2-deoxyglucose, or alpha-methyl glucoside, which are potent repressors in the wild type. Intracellular cyclic AMP levels were higher in the mutant in both the absence and the pres...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 34 2 شماره
صفحات -
تاریخ انتشار 1977