Ca-sensitive Na transport in sheep omasum.

Characterization of Urea Transport Mechanism across the Omasum Epithelium in Sheep

Sheep rumen and omasum primary cultures and source epithelia: barrier function aligns with expression of tight junction proteins.

The forestomachs of cows and sheep have historically served as important models for the study of epithelial transport. Thus, the ruminal epithelium was among the first tissues in which absorption of chloride against an electrochemical gradient was observed, requiring a tight paracellular barrier to prevent back-leakage. However, little is known about ruminal barrier function, despite the consid...

Ontogenesis of the omasum: a comparative analysis of the Merino sheep and Iberian red deer.

The aim of this study is to describe differences in the ontogenesis of the omasum in sheep (domestic ruminant) and deer (wild ruminant). A total of 50 embryos and fetuses of Merino sheep and 50 Iberian deer were used, from the first stages of prenatal life until birth. For the study, the animals were divided into five experimental groups according to the most relevant histological characteristi...

Mechanisms of intestinal phosphate transport in small ruminants.

In order to study the localization and mechanisms of intestinal phosphate transport in sheep and goats, unidirectional inorganic phosphate (Pi) flux rates across isolated stripped epithelial tissues were measured in vitro by applying the Ussing-chamber technique. In the first experiment the tissues were obtained from animals which had been kept on an adequate dietary P supply. In the second exp...

Tissue distribution of a peptide transporter mRNA in sheep, dairy cows, pigs, and chickens.

A 446-bp cDNA fragment encoding a peptide transport protein was cloned from sheep omasum and used as a probe to study the distribution of the peptide transport protein mRNA in various tissues of sheep, dairy cows, pigs, and chickens. Because the predicted amino acid sequence of this fragment was 85.8, 90.5, and 90.5% identical to rabbit, human, and rat intestinal peptide transporter (PepT1), re...