Complete Genome Sequence of a Metronidazole-Resistant Helicobacter pylori Strain
نویسندگان
چکیده
We report here the complete genome sequence of a metronidazole-resistant Helicobacter pylori strain (MET(r)). The MET(r) strain was obtained under exposure of H. pylori 26695 on agar plates with low metronidazole concentrations. The genome data provide insight into the genomic changes of H. pylori under selection by metronidazole in vitro.
منابع مشابه
The role of the rdxA gene in the evolution of metronidazole resistance in Helicobacter pylori.
It was recently demonstrated that inactivation of the rdxA gene, which encodes an oxygen-insensitive NADPH nitroreductase, is associated with the development of resistance to metronidazole by Helicobacter pylori. In order to further evaluate the contribution of rdxA to metronidazole resistance, the sequence of the rdxA gene was determined for a series of metronidazole-sensitive and -resistant i...
متن کاملDNA sequence analysis of rdxA and frxA from 12 pairs of metronidazole-sensitive and -resistant clinical Helicobacter pylori isolates.
We previously reported that inactivation of rdxA and/or frxA converted Helicobacter pylori from metronidazole sensitive to metronidazole resistant. To examine the individual roles of rdxA and frxA in the development of metronidazole resistance in H. pylori, we examined the status of rdxA and frxA from 12 pairs of metronidazole-sensitive and -resistant H. pylori isolates obtained following unsuc...
متن کاملInvestigation of frxA gene mutations in metronidazole resistant Helicobacter pylori strains in Tabriz City
Background: Due to the high rate of Helicobacter pylori infection in our country and its treatment failure, and also because of the high prevalence of antibiotic resistance of this bacterium, evaluating causes of Helicobacter pylori resistance to metronidazole and its effect on the therapeutic course is necessary. Materials and methods: A biopsy sample was obtained from 275 suspected patients r...
متن کاملComplete Genome Sequence of Helicobacter pylori Strain 7C Isolated from a Mexican Patient with Chronic Gastritis
Helicobacter pylori-induced gastritis is a risk factor for developing gastric pathologies. Here, we report the complete genome sequence of a multidrug-resistant H. pylori strain isolated from a chronic gastritis patient in Mexico City, Mexico. Nonvirulent VacA and cag-pathogenicity island (PAI) genotypes were found, but the presence of a potential mobilizable plasmid carrying an IS605 element i...
متن کاملMutations of the Helicobacter pylori genes rdxA and pbp1 cause resistance against metronidazole and amoxicillin.
To investigate amoxicillin and metronidazole resistance of Helicobacter pylori, we compared putative resistance genes between resistant strains obtained in vitro and their sensitive parent strain. All metronidazole-resistant strains had rdxA mutations, and an amoxicillin-resistant strain had pbp1 and pbp2 mutations. By transforming PCR products of these mutated genes into antibiotic-sensitive s...
متن کامل