[Frontiers in Bioscience 8, d117-134, January 1, 2003] 117 RETROVIRAL MUTATION RATES AND REVERSE TRANSCRIPTASE FIDELITY
نویسندگان
چکیده
1. Abstract 2. Importance of retroviral genetic variation 3. Historic developments in understanding retroviral genetic variation and RT fidelity 4. Factors that influence retroviral mutation rates 4.1. Reverse transcription 4.2. Other viral proteins 4.3. Nucleotide pools, DNA repair, and mammalian DNA polymerases 4.4. RNA transcription 4.5. RNA modification 4.6. Antiviral nucleoside analogs 5. In vivo fidelity assays and mutation rates 5.1. In vivo fidelity assays 5.2. In vivo mutation rates 6. In vitro fidelity assays and mutation rates 6.1. In vitro fidelity assays 6.2. In vitro mutation rates 7. Spectrum of mutations and their relative frequencies 8. Structural determinants of RT that influence fidelity 8.1. Structure of RT 8.2. Structural determinants of in vivo fidelity 8.3. Structural determinants of in vitro fidelity 8.4. The role of MLV RNase H primer grip and template-primer structure in fidelity 9. Future directions 10. Acknowledgements 11. References
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