MnO2 nanosheet mediated "DD-A" FRET binary probes for sensitive detection of intracellular mRNA.

MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc03162e Click here for additional data file.

Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles.

Optical imaging of gene expression through the use of fluorescent antisense probes targeted to the mRNA has been an area of great interest. The main obstacles to developing highly sensitive antisense fluorescent imaging agents have been the inefficient intracellular delivery of the probes and high background signal from unbound probes. Binary antisense probes have shown great promise as mRNA im...

A sensitive turn-on fluorescent probe for intracellular imaging of glutathione using single-layer MnO2 nanosheet-quenched fluorescent carbon quantum dots.

A sensitive turn-on fluorescent sensor based on single-layer MnO2 nanosheet-quenched fluorescent carbon quantum dots has been developed for rapid and selective sensing of glutathione in aqueous solutions, as well as in living cells.

Design and characterization of two-dye and three-dye binary fluorescent probes for mRNA detection.

We report the design, synthesis and characterization of binary oligonucleotide probes for mRNA detection. The probes were designed to avoid common problems found in standard binary probes such as direct excitation of the acceptor fluorophore and overlap between the donor and acceptor emission spectra. Two different probes were constructed that contained an array of either two or three dyes and ...

Highly sensitive and selective strategy for microRNA detection based on WS2 nanosheet mediated fluorescence quenching and duplex-specific nuclease signal amplification.

MicroRNAs (miRNAs) play vital roles in physiologic and pathologic processes and are significant biomarkers for disease diagnostics and therapeutics. However, rapid, low-cost, sensitive, and selective detection of miRNAs remains a challenge because of their short length, sequence homology, and low abundance. Herein, we report for the first time that WS2 nanosheet can exhibit differential affinit...