Detection of microsporidia in travelers with diarrhea.
نویسندگان
چکیده
We examined stool specimens of 148 returning travelers from an outpatient department for tropical diseases for the appearance of microsporidia using light microscopy and PCR. Intestinal microsporidiosis was diagnosed for five patients by light microscopy and for nine patients by PCR. Some cases were diagnosed only by PCR, indicating that the true prevalence has to be determined by highly sensitive techniques, such as PCR.
منابع مشابه
Identification of Encephalitozoon intestinalis in travelers with chronic diarrhea by specific PCR amplification.
With the use of Weber's modified trichrome and Uvitex 2B techniques, spores of microsporidia were detected in the stools of four travelers presenting clinically with chronic diarrhea. The general health of these patients was not impaired, and human immunodeficiency virus screening was negative. Immune evaluation, including the study of lymphocytic subpopulations, assay of serum immunoglobulins,...
متن کاملIntestinal Microsporidiosis in Iran (Kerman): Comparison in immune-compromised patients and immune competent people with diarrhea
Background and Purpose: Gastroenteritis and the clinical profile caused by Microsporidia, an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase cha...
متن کاملA Closer Look to the Most Frequent Travelers’ Disease: A Systematic Update on Travelers’ Diarrhea
The present study, wants to highlight and review the most prevalent disease amongst travelers. In the current review, an updated review regarding epidemiology, involved pathogens, and a brief review of current evidence-based guidelines for prevention and treatment of this disease are provided. A distinguishing feature of the current review is the discussion of the impacts of irritable bowel syn...
متن کاملDiarrhea Among International Travelers in Bali-Indonesia: Clinical and Microbiological Finding
Introduction: Diarrhea is a common illness among travelers to developing countries. Located in a tropical region, Bali has a potentially high prevalence of travelers diarrhea. This hospital-based cross-sectional study was carried out to assess the clinical and microbiological profiles of diarrhea among travelers admitted to Kasih Ibu hospital, Denpasar-Bali. Metho...
متن کاملDiscrimination between viable and dead Encephalitozoon cuniculi (Microsporidian) spores by dual staining with sytox green and calcofluor white M2R.
Microsporidia are obligate intracellular parasites, recognized as causing chronic diarrhea and systemic disease in AIDS patients, organ transplant recipients, travelers, and malnourished children. Species of microsporidia that infect humans have been detected in drinking-water sources, and methods are needed to ascertain if these microsporidia are viable and capable of causing infections. In th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 39 4 شماره
صفحات -
تاریخ انتشار 2001