Monokine Regulates Colony - Stimulating Activity Production by Vascular Endothelial Cells
نویسندگان
چکیده
Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colonystimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal. T-Iymphocyte-depleted. phagocytedepleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contamed a nondialyzable. heat labile factor that enhanced 4-1 5-fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69%. Lactoferrin did not inhibit
منابع مشابه
A monokine regulates colony-stimulating activity production by vascular endothelial cells.
Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal, T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (...
متن کاملVascular endothelium as a regulator of granulopoiesis: production of colony-stimulating activity by cultured human endothelial cells.
Colony-stimulating activity is a regulatory factor(s) that promotes differentiation of hemopoietic stem cells to mature granulocytes and macrophages; in man it has been found that blood monocytes, lymphocytes, and tissue macrophages produce it. In an effort to identify other potenitally physiologic tissue sources of colony-stimulating activity, we have studied the capacity of primary cultures o...
متن کاملA monokine stimulates production of human erythroid burst-promoting activity by endothelial cells in vitro.
Conditioned media were prepared from human peripheral blood monocytes and human umbilical vein endothelial cells. These media were assayed for erythroid burst-promoting activity (BPA) using human peripheral blood monocyte-depleted mononuclear cells as targets and assessing the stimulatory effect of the conditioned media on growth of early erythroid progenitor cells. Both monocytes and endotheli...
متن کاملRegulation of colony-stimulating activity production. Interactions of fibroblasts, mononuclear phagocytes, and lactoferrin.
Neonatal skin fibroblasts were cultured in supernatants of peripheral blood monocytes that had been cultured with and without lactoferrin. Granulocyte-monocyte colony-stimulating activity (CSA) was measured in supernatants of the fibroblast cultures with normal T lymphocyte-depleted, phagocyte-depleted, low density bone marrow target cells in colony growth (colony-forming unit granulocyte/macro...
متن کاملInterleukin 1 stimulates fibroblasts to produce granulocyte-macrophage colony-stimulating activity and prostaglandin E2.
Granulocyte-macrophage colony-stimulating activity (GM-CSA) can be produced by a variety of normal cell types including mononuclear phagocytes, activated T lymphocytes, endothelial cells, and fibroblasts. Recent evidence shows that a major role of the monocyte-macrophage is the recruitment of environmental cells, i.e., fibroblasts, to produce GM-CSA. In this study we have identified interleukin...
متن کامل