Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen
نویسندگان
چکیده
We proposed to measure the metabolic rate of oxygen (MRO(2)) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO(2) by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO(2) were further calculated. The measured blood flow rates in the two vessels agreed with each other.
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