Dynamics of Molecular Evolution and Phylogeography of Barley yellow dwarf virus-PAV
نویسندگان
چکیده
Barley yellow dwarf virus (BYDV) species PAV occurs frequently in irrigated wheat fields worldwide and can be efficiently transmitted by aphids. Isolates of BYDV-PAV from different countries show great divergence both in genomic sequences and pathogenicity. Despite its economical importance, the genetic structure of natural BYDV-PAV populations, as well as of the mechanisms maintaining its high diversity, remain poorly explored. In this study, we investigate the dynamics of BYDV-PAV genome evolution utilizing time-structured data sets of complete genomic sequences from 58 isolates from different hosts obtained worldwide. First, we observed that BYDV-PAV exhibits a high frequency of homologous recombination. Second, our analysis revealed that BYDV-PAV genome evolves under purifying selection and at a substitution rate similar to other RNA viruses (3.158×10(-4) nucleotide substitutions/site/year). Phylogeography analyses show that the diversification of BYDV-PAV can be explained by local geographic adaptation as well as by host-driven adaptation. These results increase our understanding of the diversity, molecular evolutionary characteristics and epidemiological properties of an economically important plant RNA virus.
منابع مشابه
Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli
متن کامل
فراوانی برخی سروتیپ‌های ویروس‌های عامل کوتولگی زرد و تغییرات فصلی جمعیت ناقلین آن‌ها در گندم و جو در شهرکرد
بیماری‌های ویروسی غلات، دارای ناقل طبیعی، در استان چهارمحال و بختیاری اغلب باعث خسارت اقتصادی می‌شوند. گونه‌های Rhopalosiphum padi، Sipha elegans، Schizaphis graminum، Sitobion avenae، Rhopalosiphum maidis و Metopolophium dirhodum به ترتیب فراوانی جمعیت به‌عنوان مهم‌ترین ناقلین ویروس‌های کوتولگی زرد جو و غلات در شهرکرد طی سال‌های 87-1385 شناخته شدند. سروتیپ های barley yellow dwarf virus-PAV (PA...
متن کاملBarley yellow dwarf virus and Cereal yellow dwarf virus Quantification by Real-Time Polymerase Chain Reaction in Resistant and Susceptible Plants.
ABSTRACT Reliable detection and quantification of barley and cereal yellow dwarf viruses (YDVs) is a critical component in managing yellow dwarf diseases in small grain cereal crops. The method currently used is enzyme-linked immunosorbent assay (ELISA), using antisera against the coat proteins that are specific for each of the various YDVs. Recently, quantitative real-time reverse-transcriptio...
متن کاملInteractions between Metopolophium festucae cerealium (Hemiptera: Aphididae) and Barley yellow dwarf virus (BYDV-PAV)
Interactions between an invasive aphid, Metopolophium festucae (Theobald) subsp. cerealium, and Barley yellow dwarf virus (BYDV-PAV) were studied under laboratory conditions. M. festucae cerealium is an economic pest of wheat and barley that has recently been found in high population densities in wheat in the Pacific Northwest of the United States. BYDV-PAV is the most prevalent and injurious s...
متن کاملImprovement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR.
One of the major factors determining the incidence of Barley yellow dwarf virus (BYDV) on autumn-sown cereals is the viruliferous state of immigrant winged aphids. This variable is assessed routinely using the enzyme-linked immunosorbant assay (ELISA). However, the threshold for virus detection by ELISA can lead to false negative results for aphids carrying less than 10(6) particles. Although m...
متن کامل