Biosynthesis of the Escherichia coli K5 capsular polysaccharide.
نویسندگان
چکیده
299-304 47 Payne, G., Virji, M. and Saunden, J. R ( I 996) in Abstracts of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., eds.), pp. 393-394, National Institutes of Health, Bethesda, MD 48 Parge, H. E.. Forest, K T.. Hickey, M. 1.. Christensen, D. A,. Getzoff, E. D. and Tainer, J. A. (I 995) Nature (London) 378, 32-38 49 Forest, K T. and Tainer, J. A. ( 1997) Gene 192, 165I69 50 Kraulis, P. J. ( I99 I ) J. Appl. Crystall. 24, 946-950 5 I Nicholls, A., Sharp, K. and Honig, B. ( I 99 I) Proteins I I , 28 1-296
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A capsular polysaccharide from Escherichia coli K5 was previously found to have the same structure, [-(4)beta GlcA(1)----(4)alpha GlcNAc(1)-]n, as that of the non-sulphated precursor polysaccharide in heparin biosynthesis [Vann, Schmidt, Jann & Jann (1981) Eur. J. Biochem. 116, 359-364]. The K5 polysaccharide was N-deacetylated (by hydrazinolysis) and N-sulphated, and was then incubated with de...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 27 4 شماره
صفحات -
تاریخ انتشار 1999