Simultaneous Separation of Ciprofloxacin, Norfloxacin and Ofloxacin by Micellar Electrokinetic Chromatography
نویسندگان
چکیده
Este trabalho descreve o desenvolvimento de um método simples de eletroforese capilar (EC) para determinação simultânea dos três derivados mais frequentemente usados da fluoroquinolona: ciprofloxacina, norfloxacina e ofloxacina. Devido às características estruturais e físico-químicas muito semelhantes, ciprofloxacina e norfloxacina não podem ser separadas pelo método clássico de eletroforese capilar de zona (CZE). Suas separações podem ser resolvidas usando cromatografia eletrocinética micelar (MEKC), adicionando um surfactante aniônico à solução-tampão. A eficiência da separação pode ser intensificada através da otimização de parâmetros analíticos. Um método rápido e confiável foi desenvolvido, e os melhores resultados foram obtidos usando uma soluçãotampão contendo tetraborato de sódio 25 mmol L, dodecilsulfato de sódio 100 mmol L e metanol 5% como modificador orgânico.
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