A Single Residue in Transmembrane Domain 11 Defines the Different Affinity
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چکیده
7 María Castañeda-Bueno, Norma Vázquez, 1 Ismael Bustos-Jaimes, 2 Damian Hernández, 1 8 Erika Rodríguez-Lobato, 1 Diana Pacheco-Alvarez, 3 Raquel Cariño-Cortés, 4 9 Erika Moreno, Norma A. Bobadilla, and Gerardo Gamba 10 11 12 Molecular Physiology Unit, 13 Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14 Instituto Nacional de Cardiología Ignacio Chávez, 15 and Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México 16 Tlalpan 14000, Mexico City, Mexico, 17 18 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de 19 México, Coyoacan, Mexico City Mexico, 20 21 Escuela de Medicina, Universidad Panamericana, Mexico City, Mexico, 22 23 Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo. Pachuca, 24 Hidalgo, México 25 26 27 Running title: Comparative analysis of rat and flounder NCC 28 29 30 Address correspondence to: Gerardo Gamba MD, PhD 31 Molecular Physiology Unit, 32 Vasco de Quiroga No. 15, 33 Tlalpan 14000, Mexico City, Mexico. 34 Phone: 5255-5513-3868, 35 Fax 5255-5655-0382, 36 e-mail: [email protected] or [email protected] 37 38 39 Articles in PresS. Am J Physiol Renal Physiol (August 18, 2010). doi:10.1152/ajprenal.00412.2010
منابع مشابه
A single residue in transmembrane domain 11 defines the different affinity for thiazides between the mammalian and flounder NaCl transporters.
Little is known about the residues that control the binding and affinity of thiazide-type diuretics for their protein target, the renal Na(+)-Cl(-) cotransporter (NCC). Previous studies from our group have shown that affinity for thiazides is higher in rat (rNCC) than in flounder (flNCC) and that the transmembrane region (TM) 8-12 contains the residues that produce this difference. Here, an ali...
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