Evaluation of Anti-cancer Potential of Ragi Bifunctional Inhibitor (RBI) from Eleusine coracana on Human Chronic Myeloid Leukemia Cells
نویسندگان
چکیده
Finger millet (ragi), a staple food crop, is well known as a super cereal for its excellent nutritional value, long-term storage potential and ethno-medicinal characteristics. Ragi bifunctional inhibitor (RBI) from Eleusine coracana Gaertn. (ragi/finger millet) belonging to cereal alpha-amylase/protease inhibitor family, inhibits alpha-amylase and trypsin simultaneously. Several seed protease inhibitors are known for their protective as well as curative role against many types of human cancers. Recently, the anti-cancer activity of ragi seed extract on K562 chronic myeloid leukemia cells was explored. In the present study, RBI was purified from finger millet seeds by affinity chromatography followed by FPLC (Fast Protein Liquid Chromatography) size exclusion separation method. Purified RBI showed cytotoxicity against K562 chronic myeloid leukemia cells (IC50 = 20 μg/ml) but, not against normal human peripheral blood mononuclear cells. Reduction of cellular proliferation and induction of apoptosis of K562 cells by purified RBI was determined by MTT (3-4,5dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry analysis. This investigation being the first report on the anti-proliferative potential of RBI from edible ragi seeds, might provide a future preventive as well as curative natural solution for chronic myeloid leukemia. _____________________________________________________________________________________________________________
منابع مشابه
The metabolism of nitrogen, calcium and phosphorus in human adults on a poor vegetarian diet containing ragi (Eleusine coracana).
Ragi (Eleusine coracana) is a grain of considerable importance in the dietary of millions of people who inhabit the Deccan Plateau of India. It is generally raised as a dry (rain-fed) crop, though in certain areas it is also raised as an irrigated crop. The grains are usually red-brown in colour, though white-skinned varieties are also known. The grain is very small and has a diameter of only 1...
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