The Effect of Hydrogen Ion Concentration on Saponin Hemolysis
نویسنده
چکیده
The purpose of the present study has been to determine the change in the resistance of corpuscles to saponin with changes in pII., the acids employed being the common inorganic acids. Ponder (1) has shown that the rate of saponin hemolysis is increased in the presence of certain acids and has applied the equation Cl = R (C, + g) to systems in which an acid is the accelerating agent. Details of technique in preparing the red blood cell suspensions and measuring the rate of hemolysis will be found elsewhere (2,3), but to explain the significance of the terms in the equation given above, it is necessary to refer to certain features of the procedure which Ponder has adopted in his studies of hemolysis and which have been followed in a portion of this investigation. In determining the effect of an agent which either accelerates or retards the action of saponin, a time-dilution curve is first constructed which shows the relation of the time taken for complete hemolysis of a standard cell suspension to the dilution of saponin, in the absence of inhibiting or accelerating agents, the determinations being made at a constant temperature. The dilutions of saponin are usually 1 part in 10,000, 20,000, 30,000, etc. The concentrations corresponding to these dilutions (6J are expressed in the above equation by Cl, the value of which in these experiments is the number of mg. of saponin contained in the tube to which the standard red blood cell suspension is added. Upon introducing into the simple cell-saponin system an agent which hastens hemolysis, a different time-dilution curve is obtained. For a given dilution of saponin complete hemolysis of a
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