Metabolic probes for imaging endosymbiotic bacteria within toxic dinoflagellates.
نویسندگان
چکیده
Fluorescence microscopy offers an important tool for the study of complex biological phenomena such as symbiosis. Here we identify a strategy that adapts the unique differences between the secondary metabolism in host and guest symbiotic species to selectively image endosymbiotic organisms. The method is demonstrated by application to the complex symbiotic relationships in toxic marine dinoflagellates.
منابع مشابه
In situ visualization of bacterial populations in coral tissues: pitfalls and solutions
In situ visualization of microbial communities within their natural habitats provides a powerful approach to explore complex interactions between microorganisms and their macroscopic hosts. Specifically, the application of fluorescence in situ hybridization (FISH) to simultaneously identify and visualize diverse microbial taxa associated with coral hosts, including symbiotic algae (Symbiodinium...
متن کاملEvolution and Distribution of Saxitoxin Biosynthesis in Dinoflagellates
Numerous species of marine dinoflagellates synthesize the potent environmental neurotoxic alkaloid, saxitoxin, the agent of the human illness, paralytic shellfish poisoning. In addition, certain freshwater species of cyanobacteria also synthesize the same toxic compound, with the biosynthetic pathway and genes responsible being recently reported. Three theories have been postulated to explain t...
متن کاملNutritional input from dinoflagellate symbionts in reef-building corals is minimal during planula larval life stage.
Dispersion of larval offspring is of fundamental ecological importance to sessile marine organisms. Photosymbiotic planulae emitted by many reef-forming corals may travel over large distances before settling to form a new colony. It is not clear whether the metabolic requirements of these planula larvae are met exclusively with lipid and protein reservoirs inherited from the mother colony or wh...
متن کاملAssessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy.
The endosymbiotic relationship between coral hosts and dinoflagellates of the genus Symbiodinium is critical for the growth and productivity of coral reef ecosystems. Here, synchrotron radiation-based infrared microspectroscopy was applied to examine metabolite concentration differences between endosymbiotic (within the anemone Aiptasia pulchella) and free-living Symbiodinium over the light-dar...
متن کاملBacterial Endocytobionts within Endosymbiotic Ciliates in Dreissena polymorpha (Lamellibranchia: Mollusca)
This paper documents a multi-level microcosm system: Dreissena-ciliates-bacteria-viruses. In the first comprehensive investigation of endocytobionts present in the cytoplasm of endosymbiotic ciliates from mussels, bacteria are described from Conchophthirus acuminatus and an undescribed Ophryoglena sp. species which are, respectively, commensal and parasitic in the freshwater bivalve Dreissena p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 46 43 شماره
صفحات -
تاریخ انتشار 2010