Qualitative and Quantitative Analysis of Dan-Deng-Tong-Nao Capsules by HPLC-DAD-MS/MS

Dan-Deng-Tong-Nao capsules (DDTNCs), a traditional Chinese medicinal preparation consisting of Radix Salvia miltiorrhiza, Radix Pueraria lobata, Erigeron breviscapus, Rhizoma chuanxiong has been used for the treatment of apoplexy and the syndrome of apoplexy involving both collaterals and meridians caused by obstruction of collaterals by blood stasis in China. In this paper, a HPLC-DAD-MS/MS method was successfully developed for qualitative and quantitative analysis of major components in DDTNCs for the first time. Twenty-six compounds were identified by comparison of their retention times and MS spectra with those elucidated standards or recorded literatures, and six of them (danshensu, 5-hydroxymethylfurfural, 3,4-dihydroxybenzaldehyde, epicatechin, puerarin and salvianolic acid A) were simultaneously determined by HPLC-DAD quantitatively. The analytical method was fully validated and successfully applied for quantitative and fingerprint analysis of DDTNCs from fifteen different production batches, results indicated that the proposed approach was applicable for the routine analysis and quality control of DDTNCs. *Corresponding author: Jianzhong Shen-Tu, Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, 79# Qingchun Road, Hangzhou 310003, PR China, Tel: 86-571-87236537; E-mail: [email protected] Received February 19, 2016; Accepted February 29, 2016; Published June 08, 2016 Citation: Zheng Y, Hu X, Lin M, Zhai Y, Wu L, et al. (2016) Qualitative and Quantitative Analysis of Dan-Deng-Tong-Nao Capsules by HPLC-DAD-MS/MS. Mass Spectrom Purif Tech 2: 110. doi:10.4172/2469-9861.1000110 Copyright: © 2016 Zheng Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.