Neotropical Entomology

The many other insects with a restricted diet, the citrus psyllid Diaphorina citri Kuwayama, the vector Causing Which agent of the HLB , the bacterium Candidatus spp Liberibacter., is intimately Associated with symbiotic microorganisms. These mutualistic symbionts play a key role on Their host nutritional ecology, and are vertically Transmitted to the progeny. However, Despite The role symbionts play on host development and reproduction, and Opportunities of the growing exploitation of the insect-symbiont association to control insect-vectored pathogens, there are very few studies on the reproductive biology and host symbiont on the transovarial transmission. Therefore, we Aimed at Analyzing the ovary During development D. citri adulthood, and at Verifying for the mating to trigger the requirement to Initiate ovary development. Newly-Emerged D. citri females Were Grouped the virgin or mated and ovary development was Observed During adulthood. Newly-Emerged females Have immature ovaries, and ovaries without any mature eggs Remain Until females mate. Once female mates, the vitellogenesis synthesis and uptake are estimulated, Quickly and oocytes are developed. Oocytes maturation in ovarioles is metachronic, with only one oocyte at a time in Developing oogenic each cycle. Morphological observations of the reproductive system including the ovaries and spermatheca, after the first cluster of eggs is laid, Indicated D. citri is polyandrous, and May Require multiple mating to Develop additional oogenic maturation cycles.