Influence of temperature on inhibition of Micrococcus pyogenes by chloramphenicol.

assay of Keilin and Hartree (Biochem. J. (London), 42, 230, 1948) was used to measure initial and residual sugar in the carbon balance studies. Total carbon was determined by the wet carbon oxidation method (Van Slyke et al., J. Biol. Chem., 191, 299, 1951), and carbon assimilation into polysaccharide and cellular material was estimated from wet carbon oxidation of the culture samples after dialysis. As ordinarily cultivated, the lag phase of A. indicum extends over a period of 6 to 7 days; such