T-suppressor cells sensitive to cyclophosphamide and to its in vitro active derivative 4-hydroperoxycyclophosphamide control the mitogenic response of murine splenic B cells to dextran sulfate. A direct proof for different sensitivities of lymphocyte subsets to cyclophosphamide

In vitro effects of 4-hydroperoxycyclophosphamide on human immunoregulatory T subset function. I. Selective effects on lymphocyte function in T-B cell collaboration

The alkylating agent cyclophosphamide may suppress or enhance immune responses in vivo but is inactive in vitro unless metabolized by microsomal enzyme activation. 4-hydroperoxycyclophosphamide (4-HC) is a synthetic compound that is spontaneously converted in aqueous solution to the active metabolites. In this report, we examined the in vitro sensitivity of functional human T cell subsets to 4-...

THE PRODUCTION OF MURINE MONOCLONAL ANTIBODIES (MAb) DIRECTED AGAINST HUMAN T- LYMPHOCYTE SUBSETS

The production of murine monoclonal antibody (MAb) has not yet been reported in Iran. The present work describes for the first time the generation of several murine hybridoma clones secreting MAbs directed against human leukocyte surface antigens. The secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. Results indicated that of seven h...

Effects of Cyclophosphamide on T Subset Function

The alkylating agent cyclophosphamide (CYP) 1 may suppress or enhance both B and T cell-mediated immune responses in vivo but requires microsomal enzyme activation for in vitro efficacy. 4-hydroperoxycyclophosphamide (4-HC) is a synthetic compound spontaneously hydrolyzed in aqueous solution to 4-hydroxycyclophosphamide, the initial metabolite formed by microsomal activation, and which subseque...

The Immunomodulatory Effect of Cyclophosphamide on Regulation of T-Cell Subsets and Antigen Presenting Cells.

The Effect of Vitamin E and Selenium on Cyclophosphamide Detoxification in Hepatic Tissues of Mature Rats

Introduction and Objective: liver is an important body organ without which life is impossible. Cyclophosphamide is an alkylating agent widely used as an anti-cancer (“antineoplastic” or “cytotoxic”) chemotherapy drug. The present study aimed to find a suitable antioxidant and examine the effect of vitamin E and selenium on reducing the side effects of cyclophosphamide on liver as a vit...