A Sensitive High-performance Liquid Chromatography Method for Analyzing Riboflavin in Fresh Fruits and Vegetables
نویسندگان
چکیده
Riboflavln (Vitamin 82) in fresh fruits and vegetables, which is present in ng levels, was separated on a reverse-phase Altex-ultrasphere ODS column with water, methanol, and heptane sulfonic acid as the mobile phase. Ion-pairing reduced tailing of the peak. Quantity of riboflavin was based on fluorescence, excitation at 450 nm, and emission at 530 nm. INTRODUCTION Analysis of riboflavin has been improved with the use of high-performance liquid chromatography (HPLC), and several
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