Toxicity of indole-3-acetic acid combined with horseradish peroxidase on Staphylococcus aureus

نویسنده

  • Mariza P. De Melo
چکیده

We investigated the action of indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) on Staphylococcus aureus death. S. aureus is a pathogen microorganism capable of causing several diseases. S. aureus was incubated for 1.5, 3.0, and 6.0 h in the absence (control) and presence of IAA, HRP or both IAA/HRP and was then examined: Colony formations, membrane integrity and membrane depolarization. Hydrogen peroxide (H2O2) and superoxide anion (O2.) production by IAA/HRP and their roles in S. aureus death were also evaluated. The gathered data were tested by ANOVA and Tukey test, using P-.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Proposed Model for the Peroxidase-Catalyzed Oxidation of Indole-3-acetic Acid in the Presence of the Inhibitor Ferulic Acid.

Linear increments in ferulic acid concentration produce logarithmic increases in the ferulic acid-induced lag periods prior to the peroxidase-catalyzed oxidation of indole-3-acetic acid in a system containing 2,4-dichlorophenol and MnCl(2) in acetate buffer at pH 5.6. Maintaining the ratio of indole-3-acetic acid to ferulic acid constant at 100 while linearly raising the ferulic acid concentrat...

متن کامل

Peroxidase-catalyzed Oxidation of Indole-3-acetic Acid.

The aerobic oxidation of indole-3-acetic acid catalyzed by horseradish peroxidase produces 1268 nm emission characteristic of singlet oxygen. Lactoperoxidase also oxidizes indole-3-acetic acid to produce singlet oxygen, but in contrast to horseradish peroxidase, this enzyme system requires hydrogen peroxide. In both of these systems, the intensity of the 1268 nm emission is small due to quenchi...

متن کامل

Oxidation of indole-3-acetic Acid-amino Acid conjugates by horseradish peroxidase.

The stability of 21 amino acid conjugates of indole-3-acetic acid (IAA) toward horseradish peroxidase (HRP) was studied. The IAA conjugates of Arg, Ile, Leu, Tyr, and Val were oxidized readily by peroxidase. Those of Ala, beta-Ala, Asp, Cys, Gln, Glu, Gly, and Lys were not degraded and their recovery was above 92% after 1 hour incubation with HRP. A correlation between the stability of IAA conj...

متن کامل

Horseradish peroxidase: a modern view of a classic enzyme.

Horseradish peroxidase is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. Site-directed mutagenesis and directed evolution techniques are now used routin...

متن کامل

On the Role of Hydrogen Peroxide in Peroxidase Catalyzed Metabolism of Indole-3-acetic Acid

Indole-3-acetic Acid, LAA-Oxidase, Peroxidase Formation of H20 2 in the course of peroxidase catalyzed IAA metabolism was measured using the peroxide-dependent decarboxylation of [14C]glyoxylate and the oxidation of phenolic substrates giving rise to fluorescent products in IAA/HRP systems. The results agree with the recent concept of IAA metabolism which includes the formation of H20 2 in the ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011