HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Guanine exchange factor RalGDS mediates exocytosis of Weibel-Palade bodies from endothelial cells
نویسندگان
چکیده
1Department of Plasma Proteins, Sanquin Research and Landsteiner Laboratory Academic Medical Centre (AMC), University of Amsterdam, Amsterdam, The Netherlands; 2Division of Molecular Neuroendocrinology, Medical Research Council (MRC) National Institute for Medical Research, London, United Kingdom; 3Cell Biology Research Group, Robarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON; 4Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands; 5Department of Vascular Medicine AMC, University of Amsterdam, Amsterdam, The Netherlands; and 6Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory AMC, University of Amsterdam, Amsterdam, The Netherlands
منابع مشابه
Ceramide triggers Weibel-Palade body exocytosis.
The sphingolipid ceramide mediates a variety of stress responses, including vascular inflammation and thrombosis. Activated endothelial cells release Weibel-Palade bodies, granules containing von Willebrand factor (vWF) and P-selectin, which induce leukocyte rolling and platelet adhesion and aggregation. We hypothesized that ceramide induces vascular inflammation and thrombosis in part by trigg...
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Weibel-Palade bodies are endothelial cell-specific organelles, which contain von Willebrand factor (vWF), P-selectin, and several other proteins. Recently, we found that the small GTP-binding protein Ral is present in a subcellular fraction containing Weibel-Palade bodies. In the present study, we investigated whether Ral is involved in the regulated exocytosis of Weibel-Palade bodies. Activati...
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Agonist-induced release of endothelial cell specific storage granules, designated Weibel-Palade bodies (WPBs), provides the endothelium with the ability to rapidly respond to changes in its micro-environment. Originally being defined as an intracellular storage pool for von Willebrand factor (VWF), it has recently been shown that an increasing number of other components, including P-selectin, i...
متن کاملSmall GTP-binding protein Ral is involved in cAMP-mediated release of von Willebrand factor from endothelial cells.
OBJECTIVE von Willebrand factor (vWF) is synthesized by endothelial cells and stored in specialized vesicles called Weibel-Palade bodies (WPBs). Recently, we have shown that the small GTP-binding protein Ral is involved in thrombin-induced exocytosis of WPBs. In addition to Ca2+-elevating secretagogues such as histamine and thrombin, release of WPB is also observed after administration of cAMP-...
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Vascular endothelial growth factor (VEGF) not only regulates angiogenesis, vascular permeability, and vasodilation but also promotes vascular inflammation. However, the molecular basis for the proinflammatory effects of VEGF is not understood. We now show that VEGF activates endothelial cell exocytosis of WeibelPalade bodies, releasing vasoactive substances capable of causing vascular thrombosi...
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