P01.20. The effect of WIN-34B on cartilage protection and regeneration by chondrogenesis from subchondral bone in vitro and in vivo
نویسندگان
چکیده
Purpose WIN-34B is a butanol fraction extract from the mixture of two oriental herbs, the dried Lonicera japonica flowers and the root of Anemarrhena asphodeloides. In previous studies, we indentified that WIN-34B has analgesic, anti-inflammatory, gastroprotective and safety effects. In this study, we measured the major components and investigated the efficacy of WIN-34B on cartilage protection and regeneration through the chondrognesis of mesenchymal progenitor cells of subchondral bone for the treatment of osteoarthritis and development of new medicines.
منابع مشابه
Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone
We sought to determine the cartilage repair capacity of WIN-34B in the collagenase-induced osteoarthritis rabbit model and in progenitor cells from subchondral bone. The cartilage protective effect of WIN-34B was measured by clinical and histological scores, cartilage area, and proteoglycan and collagen contents in the collagenase-induced osteoarthritis rabbit model. The efficacy of chondrogeni...
متن کاملSmall Molecule-BIO Accelerates and Enhances Marrow-Derived Mesenchymal Stem Cell in Vitro Chondrogenesis
Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the ma...
متن کاملThe Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects
The discovery of induced pluripotent stem cells (iPSCs) rendered the reprogramming of terminally differentiated cells to primary stem cells with pluripotency possible and provided potential for the regeneration and restoration of cartilage defect. Chondrogenic differentiation of iPSCs is crucial for their application in cartilage tissue engineering. In this study we investigated the effect of 3...
متن کاملBioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface
The cartilage lesion resulting from osteoarthritis (OA) always extends into subchondral bone. It is of great importance for simultaneous regeneration of two tissues of cartilage and subchondral bone. 3D-printed Sr5(PO4)2SiO4 (SPS) bioactive ceramic scaffolds may achieve the aim of regenerating both of cartilage and subchondral bone. We hypothesized that strontium (Sr) and silicon (Si) ions rele...
متن کاملIn Vitro Study of the Protective Effects of Hydroalcholic Extract of Soybean against Impact of Oxidative Damage on Osteogenesis and Chondrogenesis of Mouse Limb Bud
Introduction: Oxidative stress has been implicated in the pathogenesis of various diseases affecting chondrogenesis or the function of articular cartilage. The purpose of the present study was to find the effect of soybean extract on reduction of detoriation effects of oxidativestress in embryonic chondrogenesis in vitro. Methods: In order to separate ectoderm from mesenchyme, the limb buds ...
متن کامل