Effect of TGF- 2 and Anti–TGF- 2 Antibody in a New In Vivo Rodent Model of Posterior Capsule Opacification
نویسندگان
چکیده
METHODS. An extracapsular lens extraction (ECLE) was performed in 72 Sprague–Dawley rats. At the end of the procedure, 10 L TGF2 (TGF2–treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF2 antibody (anti–TGF2– treated group), or a null control IgG4 antibody (null antibody– treated control group) was injected into the capsule. Animals were killed 3 and 14 days postoperatively. Eyes were evaluated clinically prior to euthanatization, then enucleated and processed for light microscopy and immunohistochemistry afterward. PCO was evaluated clinically and histopathologically. Student’s t-test and 2 were used to assess differences between groups.
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