The regulation of respiration during the assimilation of nitrogen in Torulopsis utilis.

Tracer Studies of Nitrogen Assimilation in Yeast

By using N(15) as a tracer the assimilation of ammonia by the yeast, Torulopsis utilis, has been studied. It has been shown that: 1. There was no measurable incorporation of N in the protein or polynucleotide purine of carbon-starved yeast. 2. When ammonia is added to nitrogen-starved yeast there is a long lag period before division begins during which the yeast rapidly synthesizes protein, thi...

Incorporation of acetate and sucrose by Torulopsis utilis.

The oxidative assimilation of carbon compounds by nonproliferating yeast cells has been studied by several workers. Winzler (1940) found that when Saccharomyces cerevisiae was allowed to oxidize sodium acetate only 66 per cent of the substrate was oxidized; the remainder was incorporated into the cell. Winzler and Baumberger (1938) found that when glucose was the substrate only 26.5 per cent of...

Genome and Transcriptome Analysis of the Food-Yeast Candida utilis

The industrially important food-yeast Candida utilis is a Crabtree effect-negative yeast used to produce valuable chemicals and recombinant proteins. In the present study, we conducted whole genome sequencing and phylogenetic analysis of C. utilis, which showed that this yeast diverged long before the formation of the CUG and Saccharomyces/Kluyveromyces clades. In addition, we performed compara...

Treatment of, and Candida utilis biomass production from shochu wastewater; the effects of maintaining a low pH on DOC removal and feeding cultivation on biomass production

Shochu wastewater (SW; alcoholic distillery wastewater) contains large amounts of organic compounds (25,000 - 60,000 COD mg/L), nitrogen (1,000 - 6,000 T-N mg/L), and phosphorus (500 - 1,000 T-P mg/L). Despite its high nutrient content, SW is highly perishable, which limits its utilization for animal feed and fertilizer. Therefore, SW is mainly treated by methane fermentation. On the other hand...

The acylamide amidohydrolase of Candida utilis: purification and properties.

Amino acid biosynthesis in Torulopsis utilis and Neurospora crassa.

The present investigation is concerned with the biosynthesis of the commonly occurring open chain amino acids and proline. The methods used were tracer techniques, including isotopic competition. Since similar studies were recently carried out with Escherichiu coli (13),l the results obtained here with Torulopsis utilis and Neurospora crassa are discussed from the point of view of comparative b...