Amperometric Biosensors for Food Quality Control. Determination of Biogenic Amines, Lactulose and Glycerol
نویسندگان
چکیده
Amperometric biosensors for the measurement of biogenic amines in fish and fruit samples, of lactulose in milk and of glycerol in wine have been developed. The covalent immobilization of enzymes as diamine oxidase (amines), ? galactosidase + fructose dehydrogenase (lactulose) and glycerokinase + glycerol-3P oxidase (glycerol) has been carried out onto polymeric supports for the realization of enzyme membranes or onto glass beads for the realisation of enzyme reactors. Pt working electrodes were used as electrochemical trasducers. For most of the biogenic amines, lactulose and glycerol a linear response uo to 5 x 10 / 10 mol/L was obtained with RSD < 5%. Sensitivity was suitable for measurement in food samples. The biosensors were used to monitor biogenic amines production in salted anchovy samples, to measure biogenic amines content in modified amosphere packaged fruits, to determine lactulose in milk samples and to follow glycerol production during alcoholic fermentation. Recovery studies and/or comparison with reference procedures demonstrated that the biosensors can be used for food quality control.
منابع مشابه
Biogenic amines determination in some traditional cheeses in West Azerbaijan province of Iran
Biogenic amines (BA) are nitrogenous compounds that possess biological activity. The source of production is the microbial decarboxylation of amino acids. This compounds are found in various types of cheese. The aim of this work was to evaluate the BA content of some traditional cheeses in West Azerbaijan province Iran. For this purpose, 70 samples of Koopeh, 10 samples of Lighvan and 5 samples...
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