Eosinophil purification from peripheral blood.
نویسندگان
چکیده
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
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عنوان ژورنال:
- Methods in molecular biology
دوره 1178 شماره
صفحات -
تاریخ انتشار 2014