Comparing sensitivity and specificity methods of chemical and baronet affinity measuring glycated hemoglobin with HPLC method

Introduction: Glycated hemoglobin level is a good estimate of the average blood glucose over a period. Therefore, the measurement accuracy is very important. The most commonly available methods for measuring HbA1C are chemistry, immunoassay, enzymatic, electrophoresis, baronet affinity and HPLC. There is still no standard way that all the laboratories do it. The aim of this study was to compare two methods of chemical and baronet affinity with HPLC method for the verification accuracy. Methods: This experimental study was done on 50 samples. Glycosylated hemoglobin test performed by the baronet affinity method by Nycocard, HPLC by Bio Rad D-10 and chemical methods by Mahsa Yaran kit. Results: By HPLC, 21 samples (42%) were normal, 4 cases (8%) were moderate and 25 (50%) were high. The mean and standard deviation and the range of HbA1C of baronet affinity, chemical and HPLC methods were 7.1±1.9 (4.4 to 10.9), 6.9±1.7 (4 to 10,6), and 8.3±2.4 (4.7 to 14.3), respectively. The sensitivity of the baronet affinity method in comparison with HPLC method was 100% and specificity was 58.6%. The sensitivity of chemical method in comparison with HPLC method was 100% and specificity was 62.1%. The correlation coefficient for both was obtained 0.633 which is statistically significant (P<0.0001). Also correlation coefficient for two methods of baronet affinity and chemical was determined 0.852, which is statistically significant. American Journal of Research Communication www.usa-journals.com Malekmahmoodi, et al., 2013: Vol 1(11) [email protected] 351 Conclusions: In this study was observed that between the two methods baronet and chemical there is a coefficient of agreement of 86,9% and even the chemical method has more specifity. Also considering the cost of this method, chemical method can be considered as a suitable method for measuring Glycosylated hemoglobin. But maybe the long-time of doing chemical method is one of the serious limitations of the laboratories to select this method.