Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12.
نویسندگان
چکیده
NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.
منابع مشابه
Characteristics of Deep-Sea Environments and Biodiversity of Piezophilic Organisms
1. Investigation of Life in a High-Pressure Environment 2. JAMSTEC Exploration of the Deep-Sea High-Pressure Environment 3. Taxonomic Identification of Piezophilic Bacteria 3.1. Isolation of Piezophiles and their Growth Properties 3.2 Taxonomic Characterization and Phylogenetic Relations 4. Biodiversity of Piezophiles in the Ocean Environment 4.1. Microbial Diversity of the Deep-Sea Environment...
متن کاملCharacteristics of Deep-Sea Environments and Biodiversity of Piezophilic Organisms
1. Investigation of Life in a High-Pressure Environment 2. JAMSTEC Exploration of the Deep-Sea High-Pressure Environment 3. Taxonomic Identification of Piezophilic Bacteria 3.1. Isolation of Piezophiles and their Growth Properties 3.2 Taxonomic Characterization and Phylogenetic Relations 4. Biodiversity of Piezophiles in the Ocean Environment 4.1. Microbial Diversity of the Deep-Sea Environment...
متن کاملPiezoresponse of the cyo-operon coding for quinol oxidase subunits in a deep-sea piezophilic bacterium, Shewanella violacea.
We have isolated the genes for quinol oxidase from a deep-sea piezophilic bacterium, Shewanella violacea. Analysis of the deduced amino acid sequences of the cyo subunits showed that this oxidase has high similarity to Escherichia coli bo-type quinol oxidase. Northern blot analysis showed that these genes are expressed at a high level when the bacterium is grown at elevated pressure. Upstream i...
متن کاملPressure regulation of soluble cytochromes c in a deep-Sea piezophilic bacterium, Shewanella violacea.
Two c-type cytochromes from the soluble fraction of a deep-sea moderately piezophilic bacterium, Shewanella violacea, were purified and characterized, and the genes coding for these cytochromes were cloned and sequenced. One of the cytochromes, designated cytochrome c(A), was found to have a molecular mass of approximately 8.3 kDa, and it contained one heme c per molecule. The other, designated...
متن کاملDraft Genome Sequence of the Deep-Sea Bacterium Shewanella benthica Strain KT99
We report the draft genome sequence of the obligately piezophilic Shewanella benthica strain KT99 isolated from the abyssal South Pacific Ocean. Strain KT99 is the first piezophilic isolate from the Tonga-Kermadec trench, and its genome provides many clues on high-pressure adaptation and the evolution of deep-sea piezophilic bacteria.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 67 9 شماره
صفحات -
تاریخ انتشار 2003