The lipid content and fatty acid composition of hatched second stage juveniles of Globodera rostochiensis and G. pallida

Globodem roswch!ens!s and G. pall!da harched (l-day-old in poraro roor diffusare) second srage juveniles 02) conrained 29.2 % and 27.2 % lipid (of dry weighr), respectively. Globodera roslOch!ens!s J2 lipid consisred of 72.8 % neutral lipid, 11.3 % free fatty acids and 15.9% non-acetic phospholipid. The neutral lipid fraction comprised 95.5 % triacylglycerides, 1.8 % diacylglycerides, 2.3 % monoacylglycerides and 0.4 % cholesterol ester. Globodera pall!daJ2 lipid consisted of 73.0 % neutral lipid, 13.2 % free fatty acids and 13.8 % n.-a. phospholipid. Total unsaturated fatty acids comprised 77.4 % and 80.6 % of total lipid in G. rosLOchiensis and G. pall!da, respectively. The two species showed similar fatty acid profiles. Twenty fatty acids were identified, ranging from C 14 to C22; the major lipid classes were composed predominantly of C20 (50-60 %) and C 18 (30-35 %) fatty acids. The three mosr abundant fatty acids found in both species were C20:4, C20: I and C 18: I, making up more than 60 % of the toral. Globodera roslOchlensis J2, which had hatched over 24 h periods during the first 2 weeks exposure ro potato root diffusate, had the same fatty acid composition as those which hatched over 96 h periods in weeks 3 and 4.