Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering
نویسندگان
چکیده
منابع مشابه
Application of small angle X-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues
ABSTRACT Background: Coherent scattering leads to diffraction effects and especially constructive interferences. Theseinterferences carry some information about the molecular structure of the tissue. As breast cancer isthe most widespread cancer in women, this project evaluated the application of small angleX-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues....
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A small angle x-ray scattering (SAXS) screening platform for fully automated protein structure analysis based on a centrifugal microfluidic LabDisk is presented. Protein sample (2 μl), dilution buffer (3 μl), and screening solution (3 μl) are split into 40 nl aliquots each (CVs < 5.5%), recombined in predefined ratios to 20 samples of 240 nl and mixed. On-Disk analysis can then be performed in ...
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Small-angle X-ray scattering (SAXS) is an established technique that provides low-resolution structural information on macromolecular solutions. Recent decades have witnessed significant progress in both experimental facilities and in novel data-analysis approaches, making SAXS a mainstream method for structural biology. The technique is routinely applied to directly reconstruct low-resolution ...
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