Simultaneous AFM/Fluorescence Imaging of Living Cells – Fluorescence-guided Force Spectroscopy Application Note
نویسندگان
چکیده
High resolution atomic force microscopy (AFM) can be performed simultaneously with optical microscopy techniques, such as fluorescence or differential interference contrast (DIC) microscopy. The combined methodologies provide complementary information about the studied sample which establishes the basis for a better understanding of physiological processes and the function of biomolecules, and allow a more detailed characterization of the structure of living cells [1]. Here we present the Agilent 5500 ILM AFM/SPM, which is an easy-to-use AFM and inverted light microscope (ILM) combination that enhances live-cell imaging under physiological conditions. The advantage of this combined setup is further demonstrated by studying fluorescence-labeled membrane receptors via force spectroscopy using antibodyfunctionalized AFM tips. Introduction
منابع مشابه
Simultaneous AFM/Fluorescence Imaging of Living Cells – Fluorescence-guided Force Spectroscopy
High resolution atomic force microscopy (AFM) can be performed simultaneously with optical microscopy techniques, such as fluorescence or differential interference contrast (DIC) microscopy. The combined methodologies provide complementary information about the studied sample which establishes the basis for a better understanding of physiological processes and the function of biomolecules, and ...
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