Strain from Genogroup II Antibodies Cross-Reactive with a Human Particles and Evaluation of Monoclonal Virus-Like Protein of a Swine Norovirus into Self-Assembly of the Recombinant Capsid
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چکیده
Published Ahead of Print 8 October 2008. 10.1128/JCM.01204-08. 2008, 46(12):3971. DOI: J. Clin. Microbiol. Juan Bárcena and Ignacio Mena Mateos, José R. Castón, Wim H. M. van der Poel, Jan Vinje, Horacio Almanza, Carolina Cubillos, Iván Angulo, Francisco Strain from Genogroup II Antibodies Cross-Reactive with a Human Particles and Evaluation of Monoclonal Virus-Like Protein of a Swine Norovirus into Self-Assembly of the Recombinant Capsid
منابع مشابه
Cross-reactivity among several recombinant calicivirus virus-like particles (VLPs) with monoclonal antibodies obtained from mice immunized orally with one type of VLP.
Human caliciviruses (HuCVs) are classified into the Norwalk-like viruses (NLV) and Sapporo-like viruses (SLV) as genera within the family CALICIVIRIDAE: The NLV genus is further classified into genogroups I and II, based on sequence similarities. To study the antigenic determinants on the HuCV capsid protein and develop new diagnostic tools for field samples, we established and characterized mo...
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Norwalk virus, a member of the family Caliciviridae, is an important cause of acute epidemic nonbacterial gastroenteritis. Norwalk and related viruses are classified in a separate genus of Caliciviridae called Norovirus, which is comprised of at least three genogroups based on sequence differences. Many of the currently available immunologic reagents used to study these viruses are type specifi...
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None of the enteric caliciviruses except Po/Sapo/GIII/Cowden/80/US replicates in cell culture, which complicates efforts to develop control strategies or to study viral replication. To develop serological assays for bovine noroviruses (BoNVs) and to determine the cross-reactivity of BoNV with human noroviruses, we generated two recombinant baculoviruses, rCV186-OH and rJNCV, to express the caps...
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Noroviruses are major etiological agents of acute viral gastroenteritis. In 2002, a GII.4 variant (Farmington Hills cluster) spread so rapidly in the human population that it predominated worldwide and displaced previous GII.4 strains. We developed and characterized a panel of six monoclonal antibodies (MAbs) directed against the capsid protein of a Farmington Hills-like GII.4 norovirus strain ...
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Norovirus (NoV) capsid proteins were expressed as virus-like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad-range cross-reactivity, reacting not only with intra-genogroup strains, but also inter-genogr...
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